![]() Note that for the purpose of reducing the size of the sample project, the original nVista movies have been cropped and downsampled spatially and temporally by a factor of 2, resulting in a sampling frequency of 10 Hz and spatial size of 538 x 474 pixels to produce the “preprocessed” movies shown in the sample project.įor nVista movies acquired on the same day, series were created to process them together. The associated behavioral movie is “Trial 33” Four 5-minutes nVista movies with 2 minutes pauses between movies were acquired. The associated behavioral movie is “Trial 29”ĭay 2: two texturally different objects (gel pad and wood block) were placed in the arena. Note that Day1 and Day2 of the sample project correspond to Day1 and Day 4 in the JoVE protocol, respectively.ĭay 1: the animal was placed in a behavioral arena devoid of any objects (Open Field)). This project is composed of data acquired in the mouse somatosensory cortex (S1), using nVista with 6f (UPenn) and a 1mm diameter x ~4.3mm long prism probe. Full methods can be found in the following JoVE video protocol and paper. One project demonstrates spatial filtering to longitudinal registration and the second demonstrates multiplane de-interleaving to cell identification. To help familiarize you with the recommended Inscopix Data Processing Software, our scientists have prepared 2 processed projects you can use to explore and understand our recommended workflow. You will need the sh, tar, gunzip and tail utilities. Install the software by running sh Inscopix+Data+Processing+1.3.0.sh. From there, you should be able to open the application as normal. ![]() You can do so by clicking on the Open button. After a short verification process, you will be asked to confirm that you want to open the application. After that open the Applications directory by double-clicking on it, right-click on Inscopix Data Processing.app and click on Open in the menu that appears. ![]() After agreeing to the end user license agreement by clicking on the Agree button, simply drag and drop the application into your Applications directory in the Finder window that appears to install the software. Installation note: Mount the Inscopix Data Processing Software disk image (.dmg) file by double-clicking on it. Select "Run" to proceed with the installation.Important: Run the Inscopix Data Processing Software installer with administrator privileges so that the software can be installed for all users. Installation note: When opening an installer, you may see a message indicating the publisher cannot be verified. ![]() Windows: Windows 7 Professional and Windows 10 Pro Inscopix Data Processing v1.3.1 Installers: Windows: Windows 7 Professional and Windows 10 Pro Inscopix Data Processing Software currently supports APIs for the following: Inscopix Data Processing Software currently supports 64-bit versions of the following operating systems. We recommend that you have at least 64GB of system memory on your machine prior to running the algorithm.) ![]() (Important: CNMFe uses significantly more system memory than PCA-ICA. Below are recommended specifications for a system to run Inscopix Data Processing Software efficiently: In order to run Inscopix Data Processing Software efficiently, your system should have access to a permanent storage device with fast read/write access and plenty of space. Sample Projects with Longitudinal and Multiplane Data ![]()
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